Progen Endonuclease ELISA

This Endonuclease ELISA kit is intended for the quantitative in vitro measurement of an endonuclease of Serratia marcescens. This assay can be used to monitor the presence of endonucleases, such as DENARASE®, DENARASE® High Salt, Benzonase®, or EndoCleave, in process-related samples during process development and quality control. The assay includes the enzyme DENARASE® as a standard to generate the standard curve. When measuring different endonucleases with this assay, for accurate quantification, correction factors may be applied or custom enzyme standards may be used.

Endonuclease assay provides ultra-sensitive detection with a limit of detection (LOD) of 4 pg/ml and a limit of quantification (LOQ) of 12 pg/ml — 15–20× more sensitive than comparable kits. The dynamic range is 32–1000 pg/ml.

The Endonuclease kit is ready-to-use with pre-coated plates and reagents included, highly reproducible, broadly applicable across different endonucleases. It supports efficient workflows (up to 50% shorter incubation compared to competitor kits).

The Endonuclease assay is designed for monitoring residual nucleases during AAV and viral vector production, supporting QC in biologics and recombinant protein manufacturing, and ensuring compliance in CDMOs and CROs. It is for research use only.

In the ELISA kit manual a reference wavelength is given. The primary measurement is taken at 450 nm, so your instrument must include a suitable 450 nm filter. While the reference wavelength (between 620 nm and 690 nm) can be used, it is not strictly required. Dual-wavelength readings can, in principle, improve accuracy by correcting for non-specific optical effects in the plate. When available, reader software will automatically subtract the reference absorbance from the 450 nm signal. However, because the plates included in the kit are of high optical quality, subtracting reference values typically does not provide a noticeable statistical advantage.

Endonuclease kits remain stable during shipping and short-term storage at room temperature but should be stored at 2–8 °C upon arrival for optimal preservation. All reagents are stress-tested to ensure stability during transport, even under challenging conditions.