Custom Cytogenetics Services

Services We Offer 

2BScientific offers comprehensive custom cytogenetic services through myTags® platform, providing researchers with novel fluorescence in situ hybridisation (FISH) probes and expert support. Their synthetic oligonucleotide-based approach delivers superior performance across a range of applications, including chromosome painting, gene mapping, supporting research in animals, plants, and microbes. The services offered include:

Comprehensive Probe Design Services:

• Custom probe development for any genome reference sequence;
• Support for target regions from single loci to full chromosomes;
• Complimentary design consultation.

Flexible Synthesis Options:

• Single Synthesis: Individual probe set designs up to 108k unique sequences;
• Indexed Synthesis: Multiple probe sets (2-10 designs) per synthesis event.

Advanced Labelling Services:

• Ready-to-use labelled probes with multiple fluorophore options; 
• Immortal amplifiable substrates for in-house labelling; 
• Compatible with standard FISH protocols and oligopaints methods.

Chromosome Analysis and Visualisation:

• Multicolour FISH for chromosome identification; 
• Chromosome painting and barcoding; 3D DNA-FISH for spatial chromosome organisation; 
• Cryo-FISH for preserved sample analysis; 
• Super-resolution compatible probes for detailed structural analysis.

Advanced Genomic Applications for gene mapping and expression analysis:

• Chromosomal rearrangement detection (translocations, duplications, deletions); 
• Scaffold assembly with visual confirmation; 
• Haplotyping analysis for meiotic crossover events; 
• RNA-FISH for gene expression studies.

Key Benefits 

Superior Performance and Specificity

• Higher accuracy than traditional methods:
  • myTags® synthetic probes demonstrate higher specificity compared to genomic-derived probes, with targeted hybridisation only to intended loci while BAC-derived probes show multiple off-target hybridisations. This precision reduces experimental noise and improves data quality.
• Advanced resolution capabilities:
  • myTags® probes enable super-resolution mapping of chromosomal spatial structures when combined with spatial super-resolution structured illumination microscopy (3D-SIM), allowing researchers to resolve complex chromatin arrangements and calculate chromatin compaction levels.

Easy Implementation

• Compatible with existing FISH protocols and equipment
• Multiple format options (immortal substrates or ready-to-use labelled probes) 
• Optimised protocols available for new and experienced users 
• Standard 3–4-week turnaround time

Faster Research Efficiency

• Reduced experimental setup time with ready-to-use options
• Streamlined ordering process with expert consultation

How to Enquire 

• Fill in the custom cytogenetics services form below.
• Our Product Managers will set up a call or send an email to understand the scope of the project, specific project requirements and the desired outcome. 
• Our Team might also need to arrange a Teams with the Manufacturer to collect more detailed information.