Custom Cytogenetics Services

Services We Offer 

2BScientific offers comprehensive custom cytogenetic services through their myTags® platform, providing researchers with cutting-edge fluorescence in situ hybridization (FISH) probes and expert support. Their synthetic oligonucleotide-based approach delivers superior performance across diverse applications, from chromosome painting to gene mapping, supporting research in animals, plants, and microbes. The services offered are:

• Comprehensive Probe Design Services
   - Custom probe development for any genome reference sequence
   - Support for target regions from single loci to full chromosomes
   - Complimentary design consultation
• Flexible Synthesis Options that includes Single Synthesis: Individual probe set designs up to 108K unique sequences or  Indexed Synthesis: Multiple probe sets (2-10 designs) per synthesis event
• Advanced Labelling Services: Ready-to-use labelled probes with multiple fluorophore options; Immortal amplifiable substrates for in-house labelling; Compatible with standard FISH protocols and Oligopaints methods
• Chromosome Analysis and Visualization like Multicolour FISH for chromosome identification; Chromosome painting and barcoding; 3D DNA-FISH for spatial chromosome organization; Cryo-FISH for preserved sample analysis; Super-resolution compatible probes for detailed structural analysis
• Advanced Genomic Applications for Gene mapping and expression analysis; Chromosomal rearrangement detection (translocations, duplications, deletions); Scaffold assembly with visual confirmation; Haplotyping analysis for meiotic crossover events; RNA-FISH for gene expression studies

Key Benefits 

Superior Performance and Specificity

• Higher Accuracy Than Traditional Methods myTags synthetic probes demonstrate higher specificity compared to genomic-derived probes, with targeted hybridization only to intended loci while BAC-derived probes show multiple off-target hybridizations. This precision reduces experimental noise and improves data quality.
• Advanced Resolution Capabilities myTags probes enable super-resolution mapping of chromosomal spatial structures when combined with spatial super-resolution structured illumination microscopy (3D-SIM), allowing researchers to resolve complex chromatin arrangements and calculate chromatin compaction levels.

Easy Implementation

• Compatible with existing FISH protocols and equipment
• Multiple format options (immortal substrates or ready-to-use labelled probes) 
• Optimised protocols available for new and experienced users Standard 3–4-week turnaround time

Faster Research Efficiency

• Reduced experimental setup time with ready-to-use options
• Streamlined ordering process with expert consultation