Custom Cytogenetics Services
Services we offer
2BScientific offers comprehensive custom cytogenetic services through their myTags® platform, providing researchers with cutting-edge fluorescence in situ hybridization (FISH) probes and expert support. Their synthetic oligonucleotide-based approach delivers superior performance across diverse applications, from chromosome painting to gene mapping, supporting research in animals, plants, and microbes. The services offered are:
- Comprehensive Probe Design Services
- Custom probe development for any genome reference sequence
- Support for target regions from single loci to full chromosomes
- Complimentary design consultation - Flexible Synthesis Options that includes Single Synthesis: Individual probe set designs up to 108K unique sequences or Indexed Synthesis: Multiple probe sets (2-10 designs) per synthesis event
- Advanced Labelling Services: Ready-to-use labelled probes with multiple fluorophore options; Immortal amplifiable substrates for in-house labelling; Compatible with standard FISH protocols and Oligopaints methods
- Chromosome Analysis and Visualization like Multicolour FISH for chromosome identification; Chromosome painting and barcoding; 3D DNA-FISH for spatial chromosome organization; Cryo-FISH for preserved sample analysis; Super-resolution compatible probes for detailed structural analysis
- Advanced Genomic Applications for Gene mapping and expression analysis; Chromosomal rearrangement detection (translocations, duplications, deletions); Scaffold assembly with visual confirmation; Haplotyping analysis for meiotic crossover events; RNA-FISH for gene expression studies
Key Benefits to the Customer
Superior Performance and Specificity
- Higher Accuracy Than Traditional Methods myTags synthetic probes demonstrate higher specificity compared to genomic-derived probes, with targeted hybridization only to intended loci while BAC-derived probes show multiple off-target hybridizations. This precision reduces experimental noise and improves data quality.
- Advanced Resolution Capabilities myTags probes enable super-resolution mapping of chromosomal spatial structures when combined with spatial super-resolution structured illumination microscopy (3D-SIM), allowing researchers to resolve complex chromatin arrangements and calculate chromatin compaction levels.
Easy Implementation
- Compatible with existing FISH protocols and equipment
- Multiple format options (immortal substrates or ready-to-use labelled probes)
- Optimized protocols available for new and experienced users Standard 3–4-week turnaround time
Faster Research Efficiency
- Reduced experimental setup time with ready-to-use options
- Streamlined ordering process with expert consultation
What the Process involves
- Customers will first fill up the custom cytogenetics services form linked here – add Form here
- PMs will setup a call or send an email to understand the scope of the project, specific project requirements and the outcome customers require
- PMs can also arrange a Teams call with the technical team for further project understanding and customisation