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VeriKine ELISAs

PBL’s VeriKine HS Human IFN-Alpha All Subtypes Elisa are Best in Class assays that are optimised and validated as per Real World applications.

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PBL Assay is a highly specialised Immunoassay manufacturer that focuses on providing Best in Class highly sensitive ELISA assays specifically for human Interferons and their subunits including Interleukin cytokine detection for Human, Mouse and Cynomologus species.

PBL’s VeriKine and VeriKine HS Elisa Kits offers a wide assay range.  What makes these assays widely relevant for researchers’ use is these assays are specifically validated for CRO use by using disease based biological samples like auto-immune disease sera, normal sera and tissue culture media (TCM) testing to validate assay developments. 

These VeriKine HS Kits help support researchers and CROs for pre-clinical to clinical applications. Most effectively, these assays can detect the lowest level of endogenous cytokines and IFNs at 1pg/ml compared to its competitors, that average around 15pg/ml because of their Lower Limit of Detection capability, mainly LLOQ.

These Elisa’s can provide batch to batch consistency, best in class LLOQ and Robust Matrix Tolerance as seen for Human IFN-Alpha all Subtypes Elisa:  

Featured Kits:

  • Human IFN-Alpha
  • Human IFN-Beta
  • Human IL-22
  • Human IL-15
  • Mouse IFN-Alpha
  • Mouse IFN-Beta
  • Cynomolgus/Rhesus IFN-Alpha Cynomolgus IFN-Beta

Human IFN-Alpha All Subtype ELISA (Cat. No. 41115)
High Sensitivity

  • Assay Standard Range: 1.95-125 pg/ml
  • Accurate low pg/ml detection of all 12 human IFN-Alpha subtypes
  • Robust matrix tolerance including normal & autoimmune disease sera, EDTA plasma, and TCM
  • Optimized for autoimmune sera and plasma samples
  • Consistent, reproducible results with < 10% inter- and < 8% intra-assay CVs Detects previously "missed" IFN-Alpha subtypes

Levels of IFN-β quantified in healthy donor, influenza patient, and multiple sclerosis (MS) patient sera

VeriKine HS-IFN-α and 12 subtypes ELISA Kit - showing levels of alpha subunits in normal human serum.

IFN-β detection in MS and Influenza patients with VeriKineHS IFN-β ELISA Kit

Levels of IFN-β quantified in healthy donor, influenza patient, and multiple sclerosis (MS) patient sera

                                                               

Subtype
LOD (pg/ml)
LOQ (pg/ml)
Subtype
LOD (pg/ml)
LOQ (pg/ml)
a1 (aD)
0.09
0.343
a2a (αA)
0.056
0.214
a4a (aM1)
0.099
0.382
a5 (aG)
0.083
0.276
a6 (ak)
0.041
0.183
a7 (aJ1)
0.112
0.423
a8 (aB2)
0.284
0.993
a10 (aC)
0.108
0.447
a14 (aH)
0.168
0.585
a16 (aWA)
0.078
0.328
a17 (al)
0.156
0.522
a21 (aF)
0.293
1.129