A431 Whole Cell Lysate EGF Stimulated
Product Sizes
500 ug
W09-000-362-500UG
About this Product
- SKU:
- W09-000-362
- Additional Names:
- Cell Lysate, Stimulated Lysate, A431 Lysate EGF Stimulated, A431 Lysate Epidermal growth factor Stimulated
- Application:
- SDS-PAGE, Western Blot
- Buffer:
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue)
- CE/IVD:
- RUO
- Concentration:
- 1 mg/ml
- Extra Details:
- Ready-to-use A431 Whole Cell Lysate EGF Stimulated produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
- Formulation:
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
- Physical State:
- Liquid
- Shipping Conditions:
- Dry Ice
- Specificity:
- The cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 uM Aprotinin,
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Lysates
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=W09-000-362
