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UniGlow[TM] - One Component Chemiluminescent Substrate

Product Sizes
100 ml
£220.00
UNIGLOW-0100-100ML
About this Product
SKU:
UNIGLOW-0100
Additional Names:
Western blot detection, chemiluminescent substrate One Component System, luminol-based chemiluminescent substrate for horseradish peroxidase (HRP) detection
Application:
ELISA, Microarray, Western Blot
CE/IVD:
RUO
Concentration:
1X
Extra Details:
Enhanced Chemiluminescence (ECL) involves the enzymatic oxidation of luminol, a process that is catalyzed by peroxidase (HRP) enzyme. In the presence of HRP and a peroxide, luminol is oxidized, and emits light. The intensity of the emitted light is proportional to the amount of HRP-bound target, allowing for the quantitative detection of proteins.
Format:
Chemiluminescent
Physical State:
Liquid - light red colored
Shipping Conditions:
Ambient
Storage Conditions:
2-8[o]C unopened. Protect from light. Desiccated.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Substrates
UniGlow™ - One Component Chemiluminescent Substrate
Optimization results of the assay reagents. a 96 well plate assay results plotted on the right axis and Chemifluorescence assay result obtained from Optimizer™ microfluidic microplate is plotted on the left axis showing lower LOD and higher dynamic range and b Chemifluorescence signal output variation with increase in PfHRP2 capture antibody (CAb) concentration (bottom axis) and increase in PfHRP2 detection antibody (DAb-HRP) concentration (top axis) at a fixed DAb-HRP concentration (0.1 ug/mL) and at a fixed CAb concentration (100.0 ug/mL), respectively. Each point represents the mean of three replicates. Fig. 4. PMID: 34567620.
Cse4 ubiquitination is reduced in a cse4 K65R mutant. (A) Protein extracts were prepared from wild-type strain (BY4741) transformed with vector (pMB433), pGAL-3HA-CSE4 (pMB1458), or pGAL-3HA-cse4 K65R (pMB1791) grown in raffinose/galactose (2%) for 4 hr to induce expression of Cse4. Agarose-TUBE1 was used to pull down tandem ubiquitin binding entities and ubiquitination levels of Cse4 were detected by western blot analysis with anti-HA antibody. Input samples were analyzed using anti-HA (Cse4) and anti-Tub2 antibodies. Asterisk shows non-modified Cse4. One Component Chemiluminescent Substrate (p/n UniGlow-0100). Figure 2. PMID: 29432128.