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TMB Prestained Red ELISA Peroxidase Substrate

Product Sizes
1000 ml
£317.00
TMBE-RED-1000-1000ML
About this Product
SKU:
TMBE-RED-1000
Additional Names:
3,3',5,5'-Tetramethylbenzidine; TMB-E; TMBE; RTMBE; R-TMB-E
Application:
ELISA
CE/IVD:
RUO
Concentration:
1X
Extra Details:
TMB (3,3',5,5'-Tetramethylbenzidine) is a colorimetric substrate for peroxidase (HRP)-based enzyme immunoassays, notably ELISAs, and is known for producing a blue color that changes to yellow upon stopping the reaction with sulfuric acid.
Physical State:
Liquid - light red colored
Shipping Conditions:
Ambient
Specificity:
pH: 4.0 +/- 0.2 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS
Storage Conditions:
2-8[o]C unopened. Protect from light. Desiccated.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Substrates
TMB Prestained Red ELISA Peroxidase Substrate
Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB Prestained Red ELISA Peroxidase Substrate (p/n TMBE-RED-0100) (Rows A-D) and TMB ELISA Peroxidase Substrate (p/n TMBE-1000) (rows E-H) were added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm. The Red TMBE has comparable background to Rockland's regular TMBE.