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Streptavidin ATTO 594 Conjugated

Product Sizes
500 ug
£243.00
S000-55-500UG
About this Product
SKU:
S000-55
Additional Names:
SA, S avidin, streptococcus avidin, streptavidin AT594, ATTO 594, ATTO-TEC 594, STREPTAVIDIN ATTO 594 Conjugated
Application:
FLISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
ATTO 594
Concentration:
1 mg/ml
Extra Details:
STREPTAVIDIN ATTO 594 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
STREPTAVIDIN ATTO 594 was prepared from chromatographically purified Streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Streptavidin. No reaction was observed against anti-Avidin.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
FluoroFinder:
Streptavidin ATTO 594 Conjugated
Atto Dye-Immunofluorescence microscopy ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Western Blot of Atto 594 Conjugated Streptavidin. Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Atto 594 Conjugated Streptavidin at 1:1,1000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Guinea Pig IgG. Other band(s): Guinea Pig IgG splice variants and isoforms.