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Streptavidin Phycoerythrin Conjugated

Product Sizes
1 ml
£325.00
S000-08-1ML
About this Product
SKU:
S000-08
Additional Names:
Streptavidin PE, Phyco Streptavidin, SA Phycoerythrin, R-Phycoerythrin, Phycoerythrin conjugated streptavidin
Application:
Bioactivity, Cell-based/Functional Assay, Detection/Quantitation, Dot Blot, ELISA, Flow Cytometry, Immunofluorescence
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
R-PE
Concentration:
0.5 mg/ml
Extra Details:
Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's affinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Phycoerythrin (PE) is a red-pigmented protein found in cyanobacteria and red algae. Phycoerythrin absorbs light blue-green/yellow light and emits lightly orange/yellow light.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
Streptavidin-Phycoerythrin was prepared from electrophoretically purified streptavidin isolated from Streptomyces avidinii conjugated to the chromophore R-Phycoerythrin. Free fluorochrome is removed by tandem molecular sieve chromatography.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
FluoroFinder:
Streptavidin Phycoerythrin Conjugated
Dot Blot of Streptavidin Phycoerythrin conjugate. Row A: BSA [10mg/mL]. Row B: BSA/Biotin [1.0mg/mL]. Column Dilutions: 100ng, 33.33ng, 11.11ng, 3.70ng, 1.23ng. Primary Antibody: Streptavidin Phycoerythrin Conjugate at 1ug/mL for 1hr at RT. Secondary Antibody: None. Blocking: BlockOut Buffer (p/n MB-073).
Cytoflow of Streptavidin-Phycoerythrin (p/n S000-08). Cells: Yeast Saccharomyces cerevisiae cells expressing surface receptor protein. [LEFT] unstained; [MIDDLE] Streptavidin-Phycoerythrin at 1:200 (negative control); [RIGHT] Streptavidin-Phycoerythrin at 1:200 with biotinylated ligand test sample.
AL-57 IgG4 and ICAM-1 bind to the activated HA form of LFA-1 on PBMCs. Binding of AL-57 IgG4 (A) and ICAM-1 (B) to PBMCs with inactivating buffer (CaCl2/MgCl2) or with activating buffer (MgCl2/EGTA) in the presence or absence of PMA (10 ng/ml) or DTT (500 uM) was determined by flow cytometric analysis as described in Materials and Methods. The concentration of each IgG or ICAM-1 used was 10 ug/ml. For AL-57 staining, Neg. Control indicates negative control with just the secondary PE-labeled streptavidin (p/n S000-08) antibody used for staining; MHM24 was used as a control that did not distinguish between the HA and LA forms of LFA-1. For ICAM-1 staining, a multimeric complex [ICAM-1/streptavidin (SV)-PE] of biotinylated human ICAM-1-Fc and PE-labeled streptavidin was used; SV-PE only indicates negative control with just the PE-labeled streptavidin used for staining. Shown here are representative histograms of samples under indicated conditions. The x-axis depicts the fluorescence intensity of individual cells, and the y-axis represents the cell number. The numbers shown are relative values of MFI with relative percentages of positive cells in parenthesis. Fig. 3. PMID: 16888085.
Anti-FLAG DyLight™ 649 conjugate - X-axis (p/n 200-343-383) and Streptavidin-PE conjugate - Y-axis (p/n S000-08) are used in FACS sorting of yeast surface display camelid VHH immunized library to isolate yeast clones expressing antibodies to a target antigen. VHH antibodies displayed on yeast cell surface are tagged with FLAG epitope. Yeast library was doubly stained with Anti-FLAG DyLight™ 649 conjugate to monitor VHH expression and biotinylated antigen/Streptavidin-PE conjugate complexes to detect VHH binding to the antigen. Double positive yeast cells were isolated using FACSaria II cell sorter.