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Streptavidin Peroxidase Conjugated

Product Sizes
1 mg
£231.00
S000-03-1MG
About this Product
SKU:
S000-03
Additional Names:
HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin
Application:
Dot Blot, Electron Microscopy, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
HRP
Concentration:
1 mg/ml
Extra Details:
Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, immunohistochemistry. Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
Streptavidin-HRP was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
FluoroFinder:
ELISA Results of Human IgG Whole Molecule Biotin Conjugated using Streptavidin-HRP. Each well was coated in duplicate with 1.0 ug of Human IgG Whole Molecule Biotin Conjugate. The working dilution is 82,800. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin-HRP (p/n S000-03) and TMB substrate (p/n TMBE-1000).
Dot Blot of Human IgG F(c) Fragment Biotin Conjugated using Streptavidin HRP. Human IgG F(c) Biotin Conjugate (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Streptavidin HRP (p/n S000-03) at 1:40,000 for 30 mins at RT. Block: BlockOut buffer (p/n MB-073) at RT for 30 mins. Exposure: 1 sec.
Western Blot of Goat anti-Glycerol Kinase Antibody Biotin Conjugated using Streptavidin HRP. Lane 1: Glycerol Kinase. Load: 50 ng per lane. Primary antibody: Glycerol Kinase Antibody Biotin Conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP Streptavidin (p/n S000-03) secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Glycerol Kinase.
Streptavidin Peroxidase Conjugated
Dot Blot Results of Streptavidin Peroxidase Conjugate. Row A: BSA-Biotin Conjugated. Row B: BSA. Sample dilutions: 1- 100ng, 2- 33.33ng, 3- 11.11ng, 4-3.7ng, 5- 1.23ng. Streptavidin Peroxidase Conjugated at 1.0ug/mL for 30mins at RT.