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Biomaterials

Human Red Blood Cells 10% Washed Pooled Cells

Product Sizes
50 ml
R407-0050-50ML
About this Product
SKU:
R407-0050
Additional Names:
Human Washed Pooled Cells, Human WPCs, Human Red Blood Cells, Human RBCs, erythrocytes
Application:
Bioactivity
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Extra Details:
Human whole blood is washed to remove the platelet rich plasma, buffy coat layer, and leukocytes (WBC). Red blood cells are supplied as a 10 percent suspension in phosphate buffered saline (PBS). Human red blood cells are useful for the titration of complement, adsorption procedures, testing for agglutinins/HA assays, and for the preparation of stroma as particulate reagents. Human red blood cells are perishable and are collected and processed upon receipt of your order. PLEASE READ: This product contains blood so may require extra documentation and/or clearances, please contact sales@2bscientific.com for further information and a more Accurate quote and lead time
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Biomaterials: Blood Products
Mammalian cell toxicity assays. (A) HMPC hemolysis assay. Red blood cells (p/n R407-0050) were diluted to 2% with PBS and then incubated with HMPC over the concentration range 0.125-64 ug/ml. (B) Cytotoxicity of HMPC towards HKC-8 cells. HKC-8 cells were treated with increasing concentrations of HMPC for 24 hours before assessing viability using a WST-1 assay. (C) Cytotoxicity of HMPC towards HepG2 cells. HepG2 cells were treated with increasing concentrations of HMPC for 24 hours before assessing viability using a WST-1 assay. All experiments were repeated twice in triplicate and data show the mean +/- standard deviation. Figure 6. PMID: 29728636.
The LapDG system regulates hemagglutination. Hemagglutination assays were performed with V. cholerae O1 El Tor A1552 (A) or O1 classical O395 (B) strains. Either wild-type (WT) or indicated deletion mutants were used. The reciprocal of the lowest fold dilution (hemagglutinin [HA] titer) at which cells were able to agglutinate red blood cells was recorded. The graphs represent the means and standard deviations of HA titers from at least three biological replicates. One-way ANOVA and Dunnett's multiple-comparison test were used to compare the mean of each mutant to WT. Mean differences with a P value of ≤0.05 were deemed significant. ***, P ≤ 0.001; ****, P ≤ 0.0001. FIG 7. PMID: 31796544.