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Biomaterials

Sheep Red Blood Cells 100% Washed Pooled Cells

Product Sizes
50 ml
£462.00
R406-0050-50ML
About this Product
SKU:
R406-0050
Additional Names:
Sheep Washed Pooled Cells, Sheep WPCs, Sheep Red Blood Cells, Sheep RBCs, sheep erythrocytes
Application:
Cell-based/Functional Assay, Immunofluorescence
Buffer:
None
CE/IVD:
RUO
Extra Details:
Sheep red blood cells are useful for the titration of complement, adsorption procedures, testing for agglutinins/HA assays, and for the preparation of stroma as particulate reagents.
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Sheep whole blood is washed to remove the platelet rich plasma, buffy coat layer, and leukocytes (WBC). After processing, the finished product is supplied as 100% red blood cells. Sheep red blood cells are perishable and are collected and processed upon receipt of your order.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Biomaterials: Blood Products
IgD-only cells have intact germinal center responses but impaired IgG1+ SLPC responses.(A) Splenic (CD19+) B cells from WT, IgM-/-, and IgD-/- mice unimmunized or 5 days after i.p. immunization with 200 uL of 10% SRBCs. (B) Quantification of germinal center (Fashi GL-7hi) cells in (A). (C) Splenocytes from mice in (A). (D) Quantification of CD138+ IgG1+ plasma cells in (C). (E) WT (IgMb+) and IgM-null (IgDa+) germinal center B cells as a percentage of live splenocytes in unimmunized and IgM+/- mice 5 days after i.p. immunization with 200 uL of 10% SRBCs. (F) WT (IgG1b+) and IgM-null (IgG1a+) switched plasma cells (CD138 +IgG1+) as a percentage of live splenocytes in IgM+/- mice unimmunized or 5 days after i.p. immunization with 200 uL of 10% SRBCs. (G) Fraction of unswitched NP-specific germinal center cells (CD19+ Fashi GL-7hi IgM/IgD+) from the IgHa locus in the spleens of Balb/c-B6 F1 and IgM+/- mice 7-8 days after i.p. immunization with 100 ug NP-RSA. (H) Fraction of IgG1+CD138+ plasma cells from the IgHa locus in Balb/c-B6 F1 and IgM+/- mice 7-8 days after i.p. immunization with 100 ug NP-RSA. (I) NP-specific IgG1a and IgG1b titers at OD = 0.2 were calculated for the mice in (G-H) by ELISA. The IgG1a to IgG1b titer ratio was calculated for each mouse, and all ratios were normalized such that the average IgG1a/IgG1b ratio in Balb/c-B6 F1 samples = 1.0. For (A-D), statistics from n = 4 unimmunized mice of each genotype and n = 3 WT, n = 6 IgM-/-, and n = 7 IgD-/- immunized mice were pooled. For (E-F), n = 5 unimmunized and n = 5 immunized mice are shown. For (G-I), n = 5 Balb/c-B6 F1 mice and n = 3 IgM+/- mice are shown. One-way ANOVA with Tukey's multiple comparisons test (B and D), a paired t test (E-F), and Welch's t test (G-I) were used to calculate p values, and mean +SEM is displayed. *p<0.05, **p<0.01, ***p<0.001. Figure 7. PMID: 29521626.
DNGR signalling promotes phagosomal ROS production. a-b, Confocal images of RAW264.7 cells transfected with empty vector or plasmid encoding C9::C7 or C9(Y7F)::C7 receptors and pulsed with zymosan (a) or dead sRBCs (b) in the presence of Nitroblue tetrazolium (NBT) (Scale bar 10 um). Quantification of ROS+ phagosomes. Data represented as mean (+/- s.e.m.) (a) or (+/- s.d.) (b) and are representative of two independent determinations (n = 2). P values determined by one-way ANOVA. c, RAW264.7 stably expressing C9 or C9(Y7F) receptors were pulsed with CellTracker DeepRed (CTDR)-labelled FP-sRBCs for 2 hrs. Percentage of CTDR+ RAW264.7 cells was quantified by flow cytometry. Data represented as mean (+/- s.d.) and are representative of two independent experiments (n = 2). d, Confocal images of RAW264.7 stably expressing C9 or C9(Y7F) receptors pulsed with dead cells in the presence of NBT for 2 hrs (scale bars 10 um). Image is a representative image of three similar images. e, RT-PCR of NADPH oxidase subunits in HEK293T. Representative of two experiments (n = 2). f, HEK293T cells stably expressing C9::C7 were challenged with zymosan-Oxyburst in the presence or absence of DPI for 1 hr. Oxyburst+ positive phagosomes were quantified across 5 fields of view (n > 100 phagosomes). Data represented as mean (+/- s.e.m.). P values were calculated by unpaired parametric test, Mann-Whitney and are representative of two independent experiments (n = 2). g, HEK293T C9::C7 cells were pulsed with zymosan-Ova (left) or transfected with plasmid encoding VENUS-SIINFEKL (right) in the presence or absence of DPI (10 uM) for 4 hrs before fixing and adding of OT-I Rag1 -/- T-cells. IFN-G Gamma was assessed by ELISA, plotted as mean (+/- s.d.) of an experimental triplicate. n.s., not significant; *P ≤ 0.05; **P ≤ 0.01. Extended Data Fig 6. PMID: 33349708.