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Sepharose[TM] Protein A

Product Sizes
25 ml
£1361.00
PA50-00-0025-25ML
About this Product
SKU:
PA50-00-0025
Additional Names:
ProA, Staphylococcus A protein, Sepharose Protein A
Application:
Immunoprecipitation, Western Blot
Buffer:
20% (v/v) Ethanol
CE/IVD:
RUO
Conjugate:
Sepharose®
Concentration:
6.0 mg/cc
Extra Details:
Sepharose™ Protein A is a suspension of beads conjugated to native protein A derived from Staphylococcus aureus. When evenly dispersed approximately 2.0 cc of suspension is equal to 1.0 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 um when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10.
Formulation:
20% (v/v) Ethanol
Physical State:
Suspension of agarose beads
Shipping Conditions:
Blue Ice
Specificity:
Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is
Storage Conditions:
2-8[o]C Do not freeze.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
Abelson kinase induces phosphorylation of TACC3 Abelson kinase induces phosphorylation of TACC3. (a) Western blot performed with phospho-tyrosine specific antibody showing Abelson-induced tyrosine phosphorylation of full-length TACC3 (lane 4). Phosphorylation signal is not present when TACC3 is expressed alone (lane 2) or with another tyrosine kinase Fyn (lane 6). Total protein was incubated with A Alpha-GFP and 20uL of a 50% slurry of sepharose Protein A (p/n PA50-00-0002) and Protein G resin prewashed with lysis buffer. FIGURE 1. PMID: 32543081.
BRD4 directly interacts with eRNAs through its tandem bromodomains. d, f, In vitro pull-down of MMP9 and CCL2 eRNAs with the indicated FLAG-tagged BRD4 proteins and g, the His-tagged tandem BDs of BRD2, BRD3, BRD4, and BRDT and the single BDs of BRG1 and BRD7 as revealed by SYBR Gold staining and SDS-PAGE/Coomassie staining analyses of the purified proteins. Cleared lysates were incubated with indicated antibodies followed by an additional incubation with Protein A Sepharose (p/n PA50-00-0002). Fig 3. PMID: 30076409.
Mutp53 and NFK KappaB interact and impact each other's binding at enhancers. a Purified wild-type and mutp53 proteins bind directly to purified NFK KappaB/p65 as revealed by immunoblot analysis with an antibody that recognizes p65. Input samples for the p53 proteins were also analyzed by immunoblot analysis with an antibody that recognizes both wild-type and mutp53. Three independent interaction assays were performed. Fig. 3. PMID: 28963538.
Sepharose™ Protein A is a suspension of beads conjugated to a fusion protein containing binding domains from protein A from Staphylococcus aureus.
Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding. Another immunoglobulin binding protein is Protein L which binds to the kappa light chain of the F(ab')2 region.