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Protein A Alkaline Phosphatase Conjugated

Product Sizes
1 mg
£267.00
PA00-05-1MG
About this Product
SKU:
PA00-05
Additional Names:
ProA, Staphylococcus A protein, ALP, Protein A Alk Phos
Application:
Dot Blot, ELISA, Immunohistochemistry, Lateral Flow Assay, Western Blot
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol
CE/IVD:
RUO
Conjugate:
AP
Concentration:
1 mg/ml
Extra Details:
Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
Formulation:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A. Protein A Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein A. No reaction was observed against anti-Protein G.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
Rapid test (lateral flow immunoassay) results of sequential serum samples from a meerkat with Mycobacterium bovis infection. In the test window, the upper line (labeled C) is an internal positive control indicating mobility of the serum sample and diluent across the membrane. The lower bands of reactivity (labeled T) visualized on samples 1-5 indicate a positive reaction to the test antigens. Relative test band strengths are given in brackets: 1 = 0 months (+); 2 = 8 months (++); 3 = 10 months (+++); 4 = 15 months (++); 5 = 17 months (+++). N = negative control. Tracheal washes collected from the same animal at time points 1 and 5 cultured positive for M. bovis. Fig 2. PMID: 19139498.
Antibody responses to multiple Mycobacterium tuberculosis-complex antigens in a meerkat with Mycobacterium bovis infection revealed by multiantigen print immunoassay (MAPIA) of sequential serum samples. Incubated with alkaline phosphatase-conjugated protein A (p/n PA00-05). Antigens are indicated in the right margin, with a band visible on each strip where antibody is present. Samples were collected at the following time points: 1 = 0 months (December 2005); 2 = 8 months (August 2006); 3 = 10 months (October 2006); 4 = 15 months (March 2007); 5 = 17 months (May 2007). N = negative control. Fig 1. PMID: 19139498.