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Antibodies

Rat Peroxidase Anti-Peroxidase (PAP) Soluble Complex

Product Sizes
5 mg
P400-0005-5MG
About this Product
SKU:
P400-0005
Additional Names:
rat Peroxidase Anti-Peroxidase Soluble Complex, rat PAP Soluble Complex, rat HRP anti-HRP Solution
Application:
Immunohistochemistry
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
HRP
Concentration:
5 mg/ml
Extra Details:
The peroxidase-anti-peroxidase (PAP) approach utilizes a complex of enzyme and antibody to facilitate amplification of signal, indirectly detecting the amount of primary antibody used in an assay. Generated in Rat, the PAP complex can be utilized in any assay that utilizes a rat primary antibody. With the addition of a secondary antibody that recognizes rat, the reporter PAP complex allows for amplification of the luminescent signal (as three molecules of enzyme are bound to every two immunoglobulins).
Formulation:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen:
Peroxidase (Horseradish)
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
This product was prepared from rat ascites by delipidation, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum and anti-Peroxidase.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody