Neutral Protease Dispase[R]
Product Sizes
50 mg
£749.00
MB-127-0050-50MG
About this Product
- SKU:
- MB-127-0050
- Additional Names:
- npr|Paenibacillus polymyxa, beta-amylase, alpha-amylase, trypsin, Bacillus subtilis, Bacillus thermoproteolyticus, Bacillus megaterium, Bacillus cereus, Streptomyces griseus, Aspergillus oryzae, Serratia, Bacillus polymyxa, Bacillolysin
- Buffer:
- None
- CE/IVD:
- RUO
- Concentration:
- 4 units/mg
- Extra Details:
- Neutral protease (Dispase®) is a non-mammalian animal origin free metallo, neutral protease. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) in cell culture since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagenase. Neutral protease is an extremely stable Zn-metalloendopeptidase that is produced by Paenibacillus polymyxa. It is involved in the generation of beta- and alpha-amylases from the large amylase precursor. Although related to trypsin, neutral protease is significantly less harmful to cells and can help prevent unwanted cell clumping without cell membrane damage after one hour incubations. Neutral protease (Dispase®) is ideal for researchers focused in Stem Cell and Biomarker Research.
- Immunogen:
- npr
- Physical State:
- Lyophilized
- Shipping Conditions:
- Ambient
- Specificity:
- Neutral protease (Dispase®) is purified and one unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using casein as the substrate. The enzyme is known to contain 1g-atom of zinc per g-mol of purified enzyme. If this zinc component is removed by chelating agents such as EDTA or EGTA, an inactive apoenzyme is obtained. Calcium has been detected in the pu
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=MB-127-0050
