Collagenase/Elastase
Product Sizes
1 each
£150.00
MB-126-0001-1EACH
About this Product
- SKU:
- MB-126-0001
- Additional Names:
- CELA1, colH, colG|Clostridium histolyticum, Bacterial collagenases, collagenase, Porcine pancreatic elastase, Protease, ColH, ColG, Chymotrypsin-like elastase family member 1, Elastase-1
- Buffer:
- None
- CE/IVD:
- RUO
- Extra Details:
- Collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types 1, 2, 3, and 4 or chromatographically purified collagenase; the latter usually combined with secondary enzymes such as elastase, hyaluronidase, etc. Elastase is a serine protease that also hydrolyzes amides and esters. It is produced in the pancreas as an inactive zymogen, and activated in the duodenum by trypsin. The following information applies to porcine elastase. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Soybean trypsin inhibitor and kallikrein inhibitor suppress proteolytic but not elastolytic activity. Collagenase is ideal for researches focused in Stem Cell and Biomarker Research.
- Immunogen:
- CELA1, colH, colG
- Physical State:
- Lyophilized
- Shipping Conditions:
- Ambient
- Specificity:
- Collagenase/Elastase is partially purified and 0.22um filtered and contains 22,500 units of collagenase type 1 and 30 units of elastase per vial. Collagenase type 1 has the original balance of collagenase, caseinase, clostripain and tryptic activities. The collagenase assay is a modification of the Mandl collagen digestion procedure wherein collagenase is incubated for five hours with native colla
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=MB-126-0001
