Ribonuclease A
Product Sizes
5 mg
£233.00
MB-113-0005-5MG
About this Product
- SKU:
- MB-113-0005
- Application:
- Western Blot
- Buffer:
- None
- CE/IVD:
- RUO
- Concentration:
- 5 mg/ml
- Extra Details:
- Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science. Bovine pancreatic RNase A was secured when the Armour & Co. (of hot dog fame) purified a kilogram of it, and gave 10 mg samples away free to any interested scientists. The ability to have a single lot of purified enzyme instantly made RNase a predominant model system for protein studies. RNase A was the model protein used to work out many spectroscopic methods for assaying protein structure, including absorbance, circular dichroism/optical rotary dispersion, Raman, EPR and NMR spectroscopy. RNase A was also the first model protein for the development of several chemical structural methods, such as limited proteolysis of disordered segments, chemical modification of exposed side chains, and antigenic recognition. Ribonuclease A is ideal for investigators involved in DNA damage and repair, cell cycle proteins, stem cells and chromatin research.
- Physical State:
- Liquid
- Shipping Conditions:
- Blue Ice
- Specificity:
- RNase A is specific for pyrimidine nucleoside linkages (Volkin and Cohn 1953). The reaction is believed to take place in two steps. In the first step, the 3',5'-phosphodiester bond is cleaved, while generating a 2',3'-cyclic phosphodiester intermediate. In the second step, the cyclic phosphodiester is hydrolyzed to a 3'-monophosphate group. The first step is nonspecific with respect to the nitroge
- Storage Conditions:
- -20[o]C unopened. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=MB-113-0005
