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Proteins and Peptides

Proteinase K (Tritirachium album limber)

Product Sizes
100 mg
£228.00
MB-112-0100-100MG
About this Product
SKU:
MB-112-0100
Additional Names:
Protease K, Endopeptidase K
Buffer:
None
CE/IVD:
RUO
Extra Details:
Proteinase K is a broad-spectrum serine protease and is able to digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
A lyophilized powder. Chromatographically purified to remove DNase and RNase activity. Stable over a wide pH range : 4.0-12.5, optimal pH 7.5-8.0, using denatured hemoglobin as substrate. Although calcium ions do not affect the enzyme activity, they do protect Proteinase K against autolysis and increase thermal stability when present at a concentration of 1-5umoles. It retains its activity in the
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Enzymes