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ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free)

Product Sizes
1 l
MB-064-1000-1L
About this Product
SKU:
MB-064-1000
Additional Names:
ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free)
Application:
ELISA
Buffer:
See application note.
CE/IVD:
RUO
Concentration:
1X
Extra Details:
ELISA Microwell Blocking Buffer with Stabilizer is designed to block ELISA microwells coated with antigens, antibodies or other ligands and to stabilize the plates for drying. ELISA stabilizer allows the user to dry and store the plates for a minimum of one (1) year without significant loss of signal.
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Storage Conditions:
2-8[o]C unopened. Protect from light. Desiccated.
Supplier:
Rockland Inc
Type:
Buffers & General Consumables: Others
ELISA results of ELISA Microwell Blocking Buffer with Stabilizer (Azide and Mercury Free) (p/n MB-064). Each well was coated in duplicate with 1.0 ug of Mouse IgG Whole Molecule. Blocking Buffer (p/n MB-064, lot#35968) (Red), Blocking Buffer reference lot# (Green), and Blocking Buffer control lot# (Blue). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Rabbit Anti-Mouse IgG, Goat anti-Rabbit HRP 1:8500, and TMB substrate.
Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n TMBE-1000) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.