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ELISA Coating Stabilizer

Product Sizes
100 ml
MB-063-0100-100ML
About this Product
SKU:
MB-063-0100
Additional Names:
ELISA Microwell Coating Stabilizer (Azide and Mercury Free), ELISA Plate Coating Stabilizer, ELISA Stabilizer
Application:
ELISA
Buffer:
See application note.
CE/IVD:
RUO
Concentration:
1X
Extra Details:
ELISA Microwell Coating Stabilizer is designed to stabilize antigens, antibodies or other ligands after attachment to the surface of microwells for use in ELISA. Stabilizer allows the user to dry and store plates for a minimum of one (1) year without significant loss of signal.
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
ELISA Plate Coating Stabilizer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Storage Conditions:
2-8[o]C unopened. Protect from light. Desiccated.
Supplier:
Rockland Inc
Type:
Buffers & General Consumables: Others
ELISA Microwell Coating Stabilizer (Azide and Mercury Free)
Rockland Immunochemicals produces a wide variety of buffers and substrates for use in ELISAs. Antigen was diluted in ELISA Microwell Coating Stabilizer (p/n MB-063-0100) added to the microwell plate and incubated overnight at 4°C. The plate was then blocked with ELISA Microwell Blocking Buffer with Stabilizer (p/n MB-064-1000) for 2 hours. The primary antibody was diluted in PBS Fish Gel Concentrate (1:10)(p/n MB-066-0100), added to the plate, and allowed to incubate 1 hour at room temperature. HRP conjugated secondary antibody was diluted in HRP Conjugate Stabilizer (p/n MB-060-0100), added to the plate, and allowed to incubate for 30 minutes at room temperature. TMB ELISA Peroxidase Substrate (p/n TMBE-1000) was added to the plate and allowed to incubate for 30 minutes at room temperature. The reaction was then stopped with 1M HCl and read at 450nm.