1X RIPA Lysis Buffer
Product Sizes
50 ml
£151.00
MB-030-0050-50ML
About this Product
- SKU:
- MB-030-0050
- Additional Names:
- 1X RIPA Lysis Buffer, 1X RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer, RIPA Buffer
- Application:
- ChIP, Immunoprecipitation, Western Blot
- Buffer:
- See application note.
- CE/IVD:
- RUO
- Concentration:
- 1X
- Extra Details:
- RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. It has long been a widely used lysis and wash buffer for small-scale affinity pull-down applications, such as immunoprecipitation, since most antibodies and protein antigens are not adversely affected by the components of this buffer. In addition, RIPA Lysis Buffer minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. The following RIPA Lysis Buffer components have the following effects: Tris-HCl is a buffering agent prevents protein denaturation, NaCl is a salt that prevents non-specific protein aggregation, IGEPAL is a non-ionic detergent to extract proteins; Na-deoxycholate and SDS are ionic detergents to extract proteins; and sodium azide is a bacteriostatic agent added to retard bacterial growth. RIPA Lysis Buffer is supplied as a ready-to-use solution that requires no preparation. We suggest that the user add protease and phosphatase inhibitors not included with this product prior to use.
- Physical State:
- Liquid
- Shipping Conditions:
- Ambient
- Specificity:
- 1X RIPA Lysis Buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Buffers & General Consumables: Others
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=MB-030-0050
