10X Tris-Borate-EDTA (TBE)
Product Sizes
4 l
£200.00
MB-019-4000-4L
About this Product
- SKU:
- MB-019-4000
- Additional Names:
- buffer containing boric acid and EDTA
- Buffer:
- See application note.
- CE/IVD:
- RUO
- Concentration:
- 10X
- Extra Details:
- TBE is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases.
- Physical State:
- Liquid
- Shipping Conditions:
- Ambient
- Specificity:
- This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
- Storage Conditions:
- RT
- Supplier:
- Rockland Inc
- Type:
- Buffers & General Consumables: Others
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=MB-019-4000
