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Assay Kits & ELISAs

Oxphos[TM] Cell Survival Assay Kit

Product Sizes
1 kit
£309.00
KLD-002-1KIT
About this Product
SKU:
KLD-002
Additional Names:
Glutathione recycling, glutathione function, and glucose dependent antioxidant capacity assay, cell viability, cell survival cellular viability, cellular survival, antioxidant assay
Application:
Bioactivity
CE/IVD:
RUO
Extra Details:
OxPhos™ is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood. Oxphos™ is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). OxPhos™ measures ME in the extracellular medium without the need for cellular lysis and extraction methods. Cell media is used in the assay, avoiding the need to lyse cells and thereby saving time and cost while preserving the ability to perform other cellular tests in the same culture system. OxPhos™ may have multiple applications in aging, oxidative stress, antioxidant screening, chemotherapy response and toxicology.
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Supplier:
Rockland Inc
Type:
Assay Kits: Cell Viability
Schematic representation of the various pathways involved in the cellular interactions of HEDS. HEDS reacts spontaneously with glutathione (GSH) or in a reaction catalyzed by glutathione-S-transferase (GST) to produce mixed disulfide (MESSG) of GSH and mercaptoethanol (ME) (Pathway 1). The mixed disulfide MESSG reacts with GSH and produces ME and GSSG by the catalytic action of glutaredoxin (GRX) (Pathway 2). The glutathione disulfide GSSG reacts with NADPH and produces GSH by the catalytic action of glutathione reductase (GRase) (Pathway 3). The conversion of GSSG to GSH i.e. GSH recycling requires NADPH recycling (NADP+ NADPH) by oxidative pentose phosphate cycle (OPPC) (Pathway 4)
OxPhos™ Cell Survival Assay for human colon cancer cells in a 6 well plate. This assay gives a linear response for mammalian cells (0; 100,000; 200,000; 400,000; 600,000; and 800,000) plated in 1mL growth medium in a six well plate with up to 15% fetal bovine serum and measured 20 hours after plating. The background (BG) O.D. is between 0.231-0.235 that is subtracted from the data for each of the cell density. A mean r2 values of 0.97 demonstrate the dynamic range up to 1 million cells. y=2.376x+0.138 r2=0.964. y=2.774x+0.158 r2=0.976. y=2.819x+0.163 r2=0.976. y=2.814x+0.164 r2=0.973. y=2.736x+0.199 r2=0.965.
OxPhos™ gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 uL) suspended in a total volume of 200 uL saline in a microfuge tube and incubated with 10 uL of reagent 6 for 2hrs. OxPhos™ is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood.
OxPhos™ gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 uL) suspended in a total volume of 200 uL saline in a microfuge tube and incubated with 10 uL of reagent 6 for 2hrs. OxPhos™ is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood.
OxPhos™ gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 uL) suspended in a total volume of 200 uL saline in a microfuge tube and incubated with 10 uL of reagent 6 for 2hrs. OxPhos™ is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood.