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Assay Kits & ELISAs

RFP Chemiluminescent Western Blot Kit

Product Sizes
1 each
KCA379-1EACH
About this Product
SKU:
KCA379
Additional Names:
dsRed|Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, RFP, rRFP, mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange, mStrawberry
Application:
Western Blot
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Red Fluorescent Protein (RFP) Western Blotting kit allows for the detection of RFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-RFP antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Format:
Chemiluminescent
Immunogen:
dsRed
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Blue Ice
Specificity:
Rockland Immunochemicals' Chemiluminescent Western Blot Kit for RFP combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of recombinant proteins containing wild type Red Fluorescent Protein (DsRed or RFP) and its variants such as mBanana, mCherry, DsRed2, E2-Crimson, mOrange, mOrange2
Storage Conditions:
See Manual
Supplier:
Rockland Inc
Type:
Assay Kits: Western Blot
RFP Western Blot: Known amounts of recombinant RFP and GFP protein (p/n 000-001-215) were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4°C prior to probing the blot with the anti-GFP monoclonal antibody (p/n 600-301-215) diluted 1:1,000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n 610-4302) was performed at a dilution of 1:20,000 for 1h at 4°C. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
Flow Diagram for RFP Chemiluminescent Western Blot Procedure
Rockland Chemiluminescent Western Blot Kit for RFP contains sufficient reagents for up to 30 mini blots at 7.5 x 8 cm2.
This product is assembled as a kit. See attached protocol or CofA for further details.