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Biomaterials

Guinea Pig Brain

Product Sizes
1 each
£220.00
GP-T040-1EACH
About this Product
SKU:
GP-T040
Application:
Cell-based/Functional Assay, Immunohistochemistry, Purification, Western Blot
CE/IVD:
RUO
Physical State:
Tissue
Shipping Conditions:
Dry Ice
Storage Conditions:
-20[o]C
Supplier:
Rockland Inc
Type:
Biomaterials: Tissues
Distribution of 5-ht7 receptor binding and mRNA in guinea-pig coronal forebrain sections dissected form guinea pig brains (p/n GP-T040). (a-d) Histological sections stained with 0.25% Cresyl violet. (e-h) Autoradiographs of total binding from same sections following incubation in 1.0 nm [3HJ-5-carboxamidotryptamine ([3H]-5-CT) in the presence of (-)-cyanopindolol and sumatriptan. (i-I) Autoradiographs from sections adjacent to those in h-n, with non-specific binding defined in the presence of 1uM 5-HT. (m-p) Autoradiographs following in situ hybridization using [35S]-UTP-labelled antisense (m, n, o) and sense (p) strand riboprobes. Darker areas correspond to higher mRNA levels. Section in panel m from a different experiment from n-p. Hybridization in ventromedial hypothalamus is dense just lateral to suprachiasmatic nuclei (arrowhead). Hybridization using sense strand probe was not above background except in hippocampal dentate gyrus (DG**). Abbreviations are defined in Table 2. Figure 5. PMID: 7647964.