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DAB Membrane Peroxidase Substrate (50X)

Product Sizes
10 ml
£175.00
DAB-10-10ML
About this Product
SKU:
DAB-10
Additional Names:
3,3',4,4'-Tetraamino-diphenyl; 3,3'-Diaminobenzidine, Dab Membrane Peroxidase Substrate, Dab Membrane HRP Substrate
Application:
Immunohistochemistry, Western Blot
CE/IVD:
RUO
Concentration:
50X
Extra Details:
DAB Membrane Peroxidase Substrate (50x) is a benzidine derivative that is oxidized by hydrogen peroxide in the presence of hemoglobin, yielding a dark-brown color of solution. This oxidation can be utilized for tissue samples by first preparing them with hydrogen peroxide. DAB Membrane Peroxidase Substrate (50x) is ideal for researchers in Immunology and Microbiology research.
Physical State:
Liquid - brown
Shipping Conditions:
Ambient
Storage Conditions:
2-8[o]C unopened. Protect from light. Desiccated.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Substrates
DAB Membrane Peroxidase Substrate (50X)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) western blot staining with anti-SARS-CoV-2 Receptor Binding Domain (RBD) monoclonal antibody for detecting S1 protein (MAB10540, RnD System) (8% gel); (A): western blot staining with anti-SARS-CoV-2 S2 monoclonal antibody (MAB10557, RnD System) (8% gel); (B): western blot staining with rabbit antibody serum (12% gel); (C): western blot staining with human convalescent serum from the COVID-19 infection (8% gel); (D): (S) labeled for spike protein, (N) labeled for nucleoprotein protein, (M) labeled for membrane protein, (E) labeled for envelope protein. Western blot was performed by using antibody serum from an immunized rabbit with visualization and with HRP-conjugated anti-rabbit IgG polyclonal antibody with 1:10.000 concentration (Cat. no. 611-103-122, Rockland, USA) and DAB (Rockland, USA). Figure 5. PMID: 34621509.