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Biomaterials

Rabbit Gamma Globulin Fraction

Product Sizes
5 g
£ POA
D610-5000-5G
About this Product
SKU:
D610-5000
Additional Names:
Plasma Gamma Globulin, Serum Gamma Globulin, Globulin Fractions, Gammaglobulin, rabbit gamma fraction
Application:
ELISA, Immunohistochemistry, SDS-PAGE, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Concentration:
60 mg/ml
Extra Details:
Rabbit Gamma Globulin Fraction consists of the fraction of blood serum which contains whole antibodies as well as other non-albumin plasma proteins. Gamma globulins can be utilized therapeutically to temporarily boost an patient's immunity (such as after an immunosuppressive infection) or to increase the likelihood of kidney transplant acceptance. Gamma Globulin Fraction can be utilized in molecular biology experiments as a control reagent.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen:
Not applicable
Physical State:
Lyophilized
Shipping Conditions:
Blue Ice
Specificity:
This product was prepared from normal Rabbit serum by a multi-step process which includes delipidation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in precipitin arcs against anti-Rabbit Serum corresponding to gamma globulins.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Biomaterials: Blood Products
SDS PAGE of Rabbit Gamma Globulin. Lane 1: Non-Reduced Rabbit Gamma Globulin. Lane 2: 5uL Opal Prestained Marker MB-210-0500. Lane 3: Reduced Rabbit Gamma Globulin. Load: 1ug per lane. Predicted/Observed size: Non-Reduced at 160kDa; Reduced at 55, 25 kDa.
Development of specific, quantitative and high-throughput assays to measure pS935 LRRK2 and total LRRK2. (A,B) Novel MSD assays to measure pS935 LRRK2 and total LRRK2 levels can detect increasing amounts of recombinant LRRK2 protein; n = 3. (C) Specific detection of pS935 LRRK2 and total LRRK2 was confirmed in WT and LRRK2 KO A549 cells with and without LRRK2 kinase inhibitor treatment (MLi-2, 500 nM, 2 h) measured by western blot analysis. (D,E) Consistent with western blot data, the LRRK2 MSD assays specifically detected pS935 LRRK2 and total LRRK2 in A549 cells; n = 3. MSD signals were normalized for protein concentration, and data are shown as mean +/- SEM with p values: one-way ANOVA with Tukey's multiple comparison test; ***p ≤ 0.001, ****p ≤ 0.0001; AU, arbitrary units. Detection antibodies (15 ul for 384-well) were added to each well diluted in TBST containing 25% MSD blocker A with rabbit (p/n D610-1000) and mouse gamma globin fraction (p/n D609-0100). Fig 1. PMID: 34145320.
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