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Biomaterials

Bovine Serum Albumin 30% Solution

Product Sizes
10 ml
BSA-30-0010-10ML
About this Product
SKU:
BSA-30-0010
Additional Names:
BOVINE SERUM ALBUMIN, BSA, BSA Blocker, BSA Blocking, BSA30
Application:
Bioactivity, ELISA, FLISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.85% (w/v) Sodium Chloride
CE/IVD:
RUO
Extra Details:
Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Sterile validated preparations may be used as a nutrient in cell and microbial culture. In restriction digests, specially prepared BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
Formulation:
0.85% (w/v) Sodium Chloride
Physical State:
Liquid
Shipping Conditions:
Blue Ice
Specificity:
BOVINE SERUM ALBUMIN 30% Solution contains a 30% solution of bovine serum albumin (Fraction V, Immunoglobulin and Protease Free) in saline with preservative and stabilizer. The product is ~30% protein as determined by Biuret assay. A clear yellow green to dark amber color is typical. This product is prepared from healthy adult animals and is processed from pooled lots of serum to ensure uniformity
Storage Conditions:
2-8[o]C Do not freeze.
Supplier:
Rockland Inc
Type:
Biomaterials: Blood Products
Bovine Serum Albumin 30% Solution
Internalization of basigin monoclonal antibodies (mAbs) in hCMEC/D3 cells. (a) Representative confocal images of selected basigin mAbs exposed to hCMEC/D3 cells for 10 and 30 min. Basigin mAbs in green and nuclei visualized by Hoechst in blue. The pictures are the maximum projection of the z-stack, and the XZ projection is below the pictures. Scale bar 30 um. (b) Quantification of intracellular spots using Cellomics Arrayscan after acid stripping and staining. The intensities are normalized to the negative control and plotted as the percentage increase in spot intensity per cell with +/- standard error of the mean (SEM). Secondary antibody diluted in PBS with 2% BSA (p/n BSA-30). Figure 6. PMID: 32884039.
Surface plasmon resonance sensorgrams representing the binding of basigin extracellular domain to captured anti-basigin monoclonal antibodies. The fitted curves used for equilibrium dissociation constant (KD) calculations were obtained using the Langmuir 1:1 binding model in the Biacore S200 evaluation software and plotted in GraphPad Prism (colored in black). (a) bin A, (b) bin D, (c) bin AD, (d) bin B, and (e) bin C. At the end of each cycle, the sensor surface was regenerated in 3 M MgCl2 for 30 s. 1 x HBS-P + with 1 mg/ml bovine serum albumin (BSA, p/n BSA-30) was used as running buffer for the kinetic analyses. mAb monoclonal antibody, RU response unit. Figure 3. PMID: 32884039.