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Blotto B Pre-Mixed

Product Sizes
500 ml
£103.00
B553-0500-500ML
About this Product
SKU:
B553-0500
Additional Names:
Blotto B Pre-Mixed, TBS, 1% non-fat dry milk, 1% BSA, 0.05% TWEEN-20, blocking reagents for western or ELISA
Application:
ELISA, Western Blot
CE/IVD:
RUO
Concentration:
1X
Extra Details:
Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to and during incubation with primary antibody.
Physical State:
Lyophilized
Shipping Conditions:
Ambient
Specificity:
This product contains highly purified immunoanalytical grade non-fat dry milk and bovine serum albumin (protease and immunoglobulin-free BSA) dissolved in a solution of Tris buffered saline.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Buffers & General Consumables: Others
Blotto B Pre-Mixed - for a 1X Solution of Immunoanalytical Grade 1% Non-Fat Dry Milk, 1% BSA in TBS with Tween-20
Detection of Src-related proteins in the developing ENS. A, Western blot of Manduca embryonic gut tissue using antibodies against inactive (pan) Src and phosphorylated Src (pSrc; anti-pY416). Both antibodies detect an ~60 kDa band (the predicted size of both MsSrc isoforms). Immunoblots were preblocked for 1 h using Blotto B solution (p/n B553-0500), incubated with anti-Src (1:2000) or anti-pSrc (1:1000) antibodies overnight at 4°C, rinsed, and incubated with secondary antibodies conjugated to horseradish peroxidase. Figure 3. PMID: 19295147.