Biotin Beta Galactosidase Conjugated
Product Sizes
1 mg
B000-17-1MG
About this Product
- SKU:
- B000-17
- Additional Names:
- Vitamin H, Coenzyme R, Vitamin B7, B Beta-galactosidase, B Beta-gal, beta-gal, Biotin Beta Galactosidase Conjugated, Biotin Beta Gal Conjugated
- Application:
- Dot Blot, ELISA, Immunohistochemistry, Western Blot
- Buffer:
- 0.05 M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol
- CE/IVD:
- RUO
- Conjugate:
- Biotin
- Concentration:
- 1 mg/ml
- Extra Details:
- Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Beta-galactosidase (beta-gal; B Beta-gal) is an enzyme that hydrolyzes B Beta-galactosides (such as lactose and various glycoproteins) into simple sugars. Beta-galactosidase is routinely used as a reporter in biochemical experiments as well as a marker for gene expression experiments in E. coli. Beta-galactosidase can be used to cleave the chemical X-gal that forms an intense blue product that can easily be quantified.
- Formulation:
- 0.05 M Potassium Phosphate, 0.001M Magnesium Chloride, 0.1M Beta-Mercaptoethanol; pH 7.8
- Physical State:
- Liquid
- Reactivities:
- Label/Epitope-tag
- Shipping Conditions:
- Blue Ice
- Specificity:
- Biotin Beta Galactosidase Conjugated was prepared from electrophoretically pure Beta Galactosidase and Biotin. Biotin Beta Galactosidase assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Beta Galactosidase (E.coli) and anti-Biotin.
- Storage Conditions:
- 2-8[o]C undiluted. Do not freeze.
- Supplier:
- Rockland Inc
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules: Small Molecules
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=B000-17
