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Proteins and Peptides

Biotin Glucose Oxidase Conjugated

Product Sizes
5 mg
£147.00
B000-07-5MG
About this Product
SKU:
B000-07
Additional Names:
Biotin Glucose Oxidase Conjugation, Glucose Oxidase Conjugated Biotin, Biotin conjugated Glucose Oxidase
Application:
Dot Blot, ELISA, Immunohistochemistry, Microarray, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-D Delta-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Biotin Glucose Oxidase Conjugated is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, and Cell Biology research.
Formulation:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
Biotin Glucose Oxidase Conjugated was prepared from chromatographically purified biotin. Biotin Glucose Oxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Small Molecules
Biotin Glucose Oxidase Conjugated
Monitoring the interaction of a target with a specific ligand: (a) a microsensor's emitted-light intensity at 660 nm during the covalent binding of a 16.7 uM SAv solution with a microsensor's bare gold surface, followed by the interaction of a 1 nM bIgG solution with the microsensor-bound SAv. () exponential-decay best-fits to the emitted-light intensities: R2(SAv) = 0.88, R2(bIgG) = 0.84. (b) a microsensor's emitted-light intensity at 660 nm during the covalent binding of a 2 uM SAv solution with the microsensor's bare gold surface, followed by the interaction of a 10 nM bGOx solution with the microsensor-bound SAv. () exponential-decay best-fits to the emitted-light intensities: R2(SAv) = 0.99, R2(bGOx) = 0.96. (c) a microsensor's emitted-light intensity at 660 nm during the interactions of 100 mM DGlu/DPBS (50 mM B BetaDGlu equivalent) and 100 mM LGlu/DPBS solutions with microsensor-bound bGOx. The specific microsensor was that shown in Figure 5(b). The LGlu solution had the same refractive index as the DGlu solution but did not bind to bGOx. Both DGlu/DPBS and LGlu/DPBS solutions produced fast emitted-light intensity changes on wash-in/wash out because they had a higher refractive index than DPBS. However, only the DGlu/DPBS solution produced a slower change in emitted-light intensity due to formation of B BetaDGlu - bGOx complex. (d) a microsensor's emitted-light intensity at 660 nm during the covalent binding of a 2 uM SAv solution with the microsensor's bare-gold surface, followed by the interaction of a 66.7 nM bIgG solution with the microsensor-bound SAv. () exponential-decay best-fits to the emitted-light intensities: R2(SAv) = 0.90, R2(bIgG) = 0.94. The sample flow rate in all experiments was ~0.2 nL/s with a solution temperature of 24.0 +/- 0.1 °C. * No signal was recorded during sample loading. Figure 5. PMID: 23443397.
Dot Blot of BIOTIN GLUCOSE OXIDASE Conjugated. Lane 1: Biotin Conjugated Glucose Oxidase. Lane 2: Glucose Oxidase. Load: 3-fold serial dilution starting at 200 ng. Primary Antibody: None. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: 1% BSA-TTBS 30 min at RT.