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Proteins and Peptides

Biotin Alkaline Phosphatase Conjugated

Product Sizes
1 mg
£187.00
B000-05-1MG
About this Product
SKU:
B000-05
Additional Names:
Biotin Alkaline Phosphatase Conjugated, Biotin alk phos Conjugated, alkaline phosphatase conjugated biotin
Application:
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol
CE/IVD:
RUO
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. Biotin Alkaline Phosphatase Conjugated is ideal for investigators in Immunology, Cancer, Neuroscience, and Cell Biology.
Formulation:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Blue Ice
Specificity:
This product was prepared from electrophoretically pure Alkaline Phosphatase and Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.
Storage Conditions:
2-8[o]C unopened. Do not freeze.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Small Molecules
Biotin Alkaline Phosphatase Conjugated
Detection of 2 nmol/L of a 48-mer synthetic oligonucleotide target (T) using the Strept2-AP conjugate, a single generation of the Strept2-AP/(biot-AP) assembly, or a single generation of the Strept/(biot-AP)k complex. A noncomplementary oligo (NC) was used as the negative control. The Strept2-AP solution used for the conventional assay and for assembling the Strept2-AP/(biotAP) architectures was 10 uL of 3.1 x 10-15 mol/uL Strept2-AP + 10 mg/mL BSA in DEA buffer; 10 min of interaction. Further details are described in the Experimental Section. Figure 3. PMID: 16618179.
Dot Blot results of Biotin Alkaline Phosphatase Conjugate. Dots are Streptavidin: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Biotin-Alk Phos at 1ug/mL in MB-070 1hr RT. Imaged with BioRad ChemiDoc, Colorimetric filter.