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Avidin Cy3 Conjugated

Product Sizes
1 mg
£198.00
A003-04-1MG
About this Product
SKU:
A003-04
Additional Names:
Avidin Cy3 Conjugated
Application:
FLISA, Flow Cytometry, Immunofluorescence
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
Cyanine 3
Concentration:
1 mg/ml
Extra Details:
Avidin Cy3 Conjugated is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
Avidin Cy3 Conjugated is electrophoretically purified avidin isolated from egg white conjugated to the chromophore Cy3.29 and purified chromatographically. A single precipitin arc was observed against rabbit anti-egg white avidin when assayed by immunoelectrophoresis.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
FluoroFinder:
Avidin CY3 Conjugated
Localization and size distribution of calvarial periosteal projecting primary afferents in the TG. (A) A cross section through the scalp at a level between Bregma and Lambda (the area where the retrograde tracer FG was injected) showing peripherin-IR nerve fibers in the periosteum and skin. Note the lack of galea innervation suggesting that afferents that innervate the periosteum do not send collaterals that innervate more dorsal scalp tissues. (B) A high magnification image of a cross section through the periosteum showing peripherin-IR afferent nerve bundles (arrows) as well as periosteal mast cells (MCs, arrowheads). (C) Drawings of horizontal TG sections showing the distribution of retrograde-labeled neurons that project to the calvarial periosteum. Note the predominantly ophthalmic (V1) distribution. (D) Size distribution of TG cells that project to the calvarial periosteum. Data is the mean +/- SEM of the cross-sectional areas of the labels cells obtained from 8 experiments. (E) Based on their cell diameters, the vast majority of the calvarial periosteal projecting cells were small or medium size. Fig. 1. PMID: 24769138.
The effects of (S)-crizotinib on osteosarcoma were produced through targeting MTH1 and activating ROS. (a, b) Detection of 8-oxod-GTP incorporation into DNA in MG63 and MNNG/HOS cells treated with DMSO, 5 umol/l (S)-crizotinib by staining with Cy3-conjugated avidin. (c) Quantification of 8-oxod-GTP incorporation into DNA: bars are mean+/-SD from three independent experiments. **P<0.01 and ***P<0.001 compared with the DMSO group, Student's t-test. (d) Quantification of data of intracellular reactive oxygen species by dichlorofluorescin fluorescence in OS cells following exposure to DMSO, 5 umol/l (S)-crizotinib alone, or combined with 5 mmol/l NAC for 24 h. Bars are mean+/-SD from three independent experiments. P>0.05, **P<0.01, Student's t-test. (e) Cell viability determined by the CCK8 assay in OS cells following exposure to DMSO, 5 mmol/l NAC, 5 umol/l (S)-crizotinib alone, or combined with 5 mmol/l NAC for 72 h. Bars are mean+/-SD from three independent experiments. **P<0.01 and ***P<0.001, Student's t-test. Fig. 6. PMID: 29420337.
A nontoxic dose of ATL induces oxidative DNA damage and activates PARP in cancer cells. (e, f) Immunofluorescent staining of cellular 8-oxoG by Cy3-conjugated avidin. PC-3 cells were treated by 10 uM ATL for 12 h (scale bar: 10 um). Nuclear 8-oxoG intensity was measured using the ImageJ software and the data were processed by the Prism software. Fig. 1. PMID: 32029902.