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Avidin Peroxidase Conjugated

Product Sizes
2 mg
A003-03-2MG
About this Product
SKU:
A003-03
Additional Names:
HRP-Avidin conjugated, Horseradish peroxidase conjugated Avidin, AvD, HRP Avidin Biotin Complex, Avidin Peroxidase Conjugated, Avidin HRP Conjugated
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
HRP
Concentration:
2 mg/ml
Extra Details:
Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the greatest known non-covalent interactions. Avidity for biotin is destroyed with heat. Horseradish Peroxidase (HRP) is an enzyme that utilizes organic peroxide compounds as electron donors. Horseradish Peroxidase naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Avidin Peroxidase Conjugated is ideal for investigators in Immunology, Cancer, Neuroscience, and Cell Biology.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
Avidin Peroxidase Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin Peroxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Avidin and anti-Peroxidase.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
Detection of 20S Proteasome Subunits Modified by Reactive Oxygen Species after 2-DE. Images were taken from a study on a differential analysis of ROS sensitive 20S proteasome subunits, which were characterized using biotin-conjugated chemical reagents to visualize the degree of oxidation. A) Free thiol groups of murine 20S proteasome subunits were conjugated with iodoacetyl-LC-Biotin and probed with avidin-HRP. In this indirect assay, diminishing signal indicates an increasing level of oxidation. Parallel detection of free thiol groups in vehicle and ROS treated murine proteasome complexes demonstrated that the A Alpha2, B Beta1, B Beta3 and B Beta5i subunits were oxidized at higher levels after ROS treatment. B) In parallel experiments, carbonylation was visualized by derivatization with 2,4-dinitrophenylhydrazine (DNPH) and probed with an anti-DNP antibody. In this direct assay, the increasing signal indicates higher levels of oxidation. Increased carbonyl modification of 20S proteasome subunits was detected for A Alpha2, A Alpha4, A Alpha6 and B Beta3 after ROS treatment. Figure 4. PMID: 19003867.
Avidin Peroxidase Conjugated