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Avidin Fluorescein Conjugated

Product Sizes
2 mg
£158.00
A003-02-2MG
About this Product
SKU:
A003-02
Additional Names:
Avidin Fluorescein Conjugated, Avidin FITC Conjugated
Application:
Bioactivity, Dot Blot, FLISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Conjugate:
FITC
Concentration:
1.1 mg/ml
Extra Details:
Avidin Fluorescein Conjugated is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Physical State:
Lyophilized
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Ambient
Specificity:
Avidin Fluorescein Conjugated was prepared from chromatographical pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Avidin. No reaction was observed against anti-Streptavidin.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules: Conjugated Protein
FluoroFinder:
Avidin Fluorescein Conjugated
Dot Blot of Avidin Fluorescein conjugated. Lane A: BSA Biotin Conjugated. Lane B: BSA. Lanes 1 load: 100ng, 2-5: serial dilution 3 fold. Primary Antibody: n/a. Secondary Antibody: Avidin Fluorescein Conjugated at 1 ug/mL at RT for 1 hour. Block: MB-070 for 30 min at RT.
BSA-FITC solution exposed to three time-averaged patterns simultaneously at 110 mW for 10 s. (a) Real-time microscope image captured during fabrication. (b) Fluorescent image of patterned protein after sample wash with 1x PBS. (c) SEM image of sample after a chemical desiccation process. Predicted feature dimensions were assessed using area measurements from both the fluorescent and SEM images. The fluorescent measurements indicated the features were ~6% larger than desired, while the SEM measurements indicated they were ~6% smaller than desired; demonstrating an accurate tolerance for pattern transfer. Next, a BSA-FITC solution spiked with avidin conjugated FITC was patterned. Biotin conjugated with ATTO 655 was applied to the resulting BSA/avidin structures for 20 min to assess the retention of avidin binding ability. Fluorescent microscope images were taken using (d) FITC and (e) Cy5 filter sets. A combined fluorescent image (f) reveals the functionality of the substrate after patterning. All scale bars are 50 um. Fig. 4. PMID: 20589036.