Mouse TrueBlot[R] Set (IP Agarose beads)
Product Sizes
1 set
£381.00
88-7788-31-1SET
About this Product
- SKU:
- 88-7788-31
- Additional Names:
- Anti-Mouse immunoglobulin Gamma, Agarose-conjugated IgG, Anti-Ms IgG, Anti-Mouse IgG HRP, TrueBlot, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot, HRP, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for western blotting, Anti-Mouse IgG IP Agarose
- Application:
- Immunoprecipitation, Western Blot
- Buffer:
- 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- translate.label.attr.clone:
- eB144
- Clonality:
- Monoclonal
- Conjugate:
- HRP
- Extra Details:
- Mouse IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Mouse IgG TrueBlot®, simply substitute the conventional HRP Anti-Mouse IgG blotting reagent with Mouse IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Mouse IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.
- Formulation:
- 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Isotype:
- IgG
- Physical State:
- Liquid
- Reactivities:
- Mouse
- Shipping Conditions:
- Blue Ice
- Specificity:
- Mouse TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA.
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Rockland Inc
- Type:
- Microspheres: Purification
- FluoroFinder:
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=88-7788-31
