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Antibodies

Fab Rabbit IgG (H&L) Antibody

Product Sizes
1 mg
£175.00
811-7102-1MG
About this Product
SKU:
811-7102
Additional Names:
Donkey Fab Anti-Rabbit IgG Antibody, Donkey Fab Fragment Anti-Rabbit IgG Antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Fab Anti-Rabbit IgG (H&L) Antibody generated in donkey detects immunoglobulin g from rabbit, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Donkey
Immunogen:
Rabbit IgG whole molecule
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Rabbit
Shipping Conditions:
Blue Ice
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum. No reaction was observed against anti-Papai
Storage Conditions:
2-8[o]C diluted.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Double labeling of Trpm4 (red) and T TauGFP (green). Trpm4 is expressed in VSNs of sexually naive male and female mice. (A) T TauGFP immunostaining (green) in a coronal cryosection of the left VNO of a 7-week-old Trpm4-IC/eR26-T TauGFP mouse reports widespread Trpm4 gene expression in sensory neurons and in supporting cells of the VNE. T TauGFP-IR is also present in cells of the non-sensory (ns) epithelium and in vascular endothelial cells. (B) Magnification of the VNE of male () and female () Trpm4-T TauGFP reporter mice show that Trpm4 protein (red) colocalizes with T TauGFP fluorescence (green) in VSNs but is absent in supporting cells. (C) In about 50% of females, VSNs were devoid of Trpm4 protein despite the presence of T TauGFP. (D) The vast majority of Trpm4+ VSNs (red) colocalizes with the olfactory marker protein (OMP, green), a marker for mature VSNs, in somata, dendrites and dendritic knobs, but not in VSN axon bundles (arrows). (E) The specificity of the Trpm4 antibody is verified by the absence of immunoreactivity in the VNO of Trpm4-/- male mice. (F) RT-PCR analysis of Trpm4 and Trpm5 mRNA prepared from whole VNO and from isolated VSNs (7-10 cells/sample) of male and female B6 and Trpm4-IC/eR26-T TauGFP mice. Sequence analysis confirmed that the 4.2 kb Trpm4 amplicon (arrowhead) in each sample encodes full-length Trpm4 mRNA. The 4.1 kb Trpm5 amplicon (arrowhead) encoding the full-length Trpm5 mRNA was only detected in whole VNO but absent in isolated VSNs. Identity of dissociated VSNs was verified by RT-PCR for olfactory marker protein (Omp). Control reactions omitting reverse transcriptase (-RT) showed no PCR products ruling out genomic DNA contamination. Scale bars (A) 200 um, (B-E) 20 um. Fig. 2. PMID: 32298804.
Trpc2 but not Trpm4 protein localizes to VSN microvilli. (A) Double labeling of Trpm4 (red) and Trpc2 (green) depicts co-localization of both TRP channels in VSN somata and dendrites (right panel). The most apical layer (arrowheads) shows robust Trpc2 labeling (green). (B) High-resolution confocal image (1-um optical section) of the dendritic endings (d) of VSNs shows that microvilli (mv) are heavily labeled for Trpc2 (green, arrowheads) whereas Trpm4-IR (red) was not detected in the microvilli. (C) Representative examples of Trpm4-IR (green) in coronal sections of VNE from B6 mice at postnatal days (P) 1, P7, P14 and P21. Trpm4 protein expression emerges at around P7. Number of Trpm4+ VSNs increases with age. (D) Quantification of Trpm4+ VSNs over developmental time as percentages of the total number of VSNs determined by nuclear Hoechst staining: P7 (33 +/- 1%, n = 2 male and 2 female mice, 13 sections, 3-4 sections/mouse); P14 (45 +/- 0.2%, n = 2 male and 2 female mice, 15 sections, 3-4 sections/mouse); P21 (71 +/- 4.5%, n = 1 male and 2 female mice, 12 sections, 4 sections/mouse). Individual data points represent the averaged cell counts obtained from a single mouse. Data are expressed as means +/- SD. Scale bars (A, B) 20 um, (C) 2 um. Fig. 3. PMID: 32298804.