Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

Fab Mouse IgG (H&L) Antibody

Product Sizes
500 ug
810-4102-500UG
About this Product
SKU:
810-4102
Additional Names:
Rabbit Fab Anti-Mouse IgG Antibody, Rabbit Fab Fragment Anti-Mouse IgG Antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Fab Anti-Mouse IgG (H&L) Antibody generated in rabbit detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Mouse IgG whole molecule
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse
Shipping Conditions:
Blue Ice
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed against anti-Papain
Storage Conditions:
2-8[o]C diluted.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Confocal images of semi-thin sections of the mouse retina triple-immunolabelled with mouse monoclonal antibodies against CASPR1, rabbit polyclonal antibodies against PSD-95 (L667) and rabbit polyclonal antibodies against RIBEYE (U2656), as described in the Materials and Methods section. In A4,A4 and B4,B5 the indicated two immunosignals are overlayered on each other; in A6, B6, all three immunosignals were overlayered on each other. ONL, outer nuclear layer; OPL, outer plexiform layer. Scale bars: 2um. Figure S7. PMID: 30266776.
CASPR1 is located pre-synaptically in close vicinity to the synaptic ribbon. A) High resolution confocal analysis of rod photoreceptor synapses in the OPL of the mouse retina (0.5um-thin sections) that were triple-immunolabelled with the rabbit polyclonal antibody against CASPR1, mouse monoclonal antibody 2D9 against RIBEYE and the indicated third primary antibodies (A-F). In (A), the outline of a single presynaptic terminal was visualized by immunolabelling with antibodies against PSD95 (A2). The CASPR1 immunosignal is located within the presynaptic terminal in close vicinity to the synaptic ribbon. Furthermore, presynaptic CASPR1 was found in close vicinity to the active zone markers RIM2 (B) and CASK (C,D,E). In contrast, the CASPR1 signal did not overlap with the postsynaptic signal for mGluR6 that is localized at the tip of invaginating ON-bipolar cells that are located also in close vicinity to the synaptic ribbon (Appendix Fig. S8F). Appendix Figs. S8B,C,D,F were obtained by confocal microscopy; Appendix Figs. S8A,E by super-resolution structured illumination-microscopy (SR-SIM). OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer. Scale bars: 1um (A-F). Fig S8. PMID: 30266776.
Semi-thin (0.5 um-thin) sections of the mouse cerebellum double-immunolabeled with the monoclonal dynamin1xb antibody and the indicated other primary antibodies. The other primary antibodies against synaptotagmin1 (A,B), synaptic vesicle protein 2 (SV2; C) and RIM1/2 (D) were applied to label the synapses in order to better relate the dynamin1xb immunosignals to the synaptic regions. We observed a strong dynamin1xb immunosignal in the cerebellar cortex whereas the cerebellar medulla (white matter) that contains predominantly fiber tracts (but no synapses) was not immunolabeled. In the cerebellar cortex, dynamin1xb was highly enriched in the synaptic regions, i.e., the molecular layer (mol) of the cerebellar cortex and the giant synapses in the granule cell layer (arrows) of the cerebellar cortex. No significant dynamin1xb immunosignal was observed in the medulla of the cerebellum that predominantly contains axonal fiber tracts. (A,B,D) was obtained by epifluorescence microscopy; (C) was obtained by confocal microscopy. Abbreviations: mol, molecular layer; Pu, Purkinje cell layer; gr, granule cell layer. Scale bars: 50 um (A-D). Fig 5. PMID: 28790889.