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Antibodies

Fab Human IgG (H&L) Antibody

Product Sizes
500 ug
£280.00
809-4102-500UG
About this Product
SKU:
809-4102
Additional Names:
Rabbit Fab Anti-Human IgG Antibody, Rabbit Fab Fragment Anti-Human IgG Antibody
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Fab Anti-Human IgG (H&L) Antibody generated in rabbit detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Human IgG whole molecule
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Blue Ice
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed against anti-Papain
Storage Conditions:
2-8[o]C diluted.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Fluorescence microscopy using Rabbit Fab Anti-Human IgG. Analysis of human IgGs displayed on the surface of OCMS™ hybridomas. (a) 8C5 OCMS™ (top row) and B5-6T OCMS™ (bottom row) cells were incubated overnight with the RAH (AB14), followed by biotinylated rabies GP and Alexa Fluor 488® streptavidin (green) (AB3), and visualized by fluorescence microscopy. Images are shown with DAPI (blue) (right) or without DAPI (left). Scale bar = 5 um. (b) The 4G4 OCMS™ (top row) and 8C5 OCMS™ (bottom row) hybridomas were incubated overnight with a rabbit Fab anti-human IgG (AB17), then for an hour with additional rabbit Fab anti-human IgG and Sabin type III PV (left column) or GP (right column), followed by an Alexa Fluor® 488 anti-human IgG (green) (AB2). Nuclei were stained with DAPI (blue). Scale bar = 10 um. (c) In this mixing experiment, non-secretor OCMS™ cell lines were labeled with CFSE (green) and mixed in a 10:1 ratio with IgG-secreting OCMS™ hybridomas. Cells were incubated overnight with rabbit F(ab')2 anti-human IgG (AB15) then washed and incubated with biotinylated Protein A (AB12) and APC streptavidin (red) (AB4). (Top row) 8C5 OCMS™ vs. B5-6T OCMS™ CFSE. (Bottom row) 4G4 OCMS™ vs. LCX OCMS™ CFSE. With DAPI (blue) (right column) or without (left column). Scale bar = 5 um. Figure 2. PMID: 30794061.