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Antibodies

F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Product Sizes
500 ug
£426.00
710-708-124-500UG
About this Product
SKU:
710-708-124
Additional Names:
Donkey F(ab')2 Anti-Mouse IgG Antibody Phycoerythrin Conjugation, Donkey Fab2 Anti-Mouse IgG PE Conjugated Antibody
Application:
Dot Blot, Flow Cytometry, Immunofluorescence
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
R-PE
Concentration:
0.5 mg/ml
Extra Details:
F(ab')2 Anti-Mouse IgG whole molecule generated in donkey detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Donkey
Immunogen:
F(ab')2 Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Mouse
Shipping Conditions:
Ambient
Specificity:
F(ab')2 fragment PE conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Seru
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
Dot Blot results of Donkey F(ab')2 Anti-Mouse IgG Antibody Phycoerythrin Conjugated. Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: Donkey F(ab')2 Anti-Mouse IgG Antibody Phycoerythrin at 1ug/mL for 1hr at RT. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Rhodamine filter.
Dot Blot of F(ab')2 Donkey Anti-Mouse IgG Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins. Antigen: Mouse IgG. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng or 1.23 ng as indicated. Primary antibody: N/A. Secondary antibody: F(ab')2 Donkey Anti-Mouse IgG Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins at 1:1,000 for 60 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 60 min at RT.