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Antibodies

F(ab')2 Goat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

Product Sizes
500 ug
£426.00
705-708-125-500UG
About this Product
SKU:
705-708-125
Additional Names:
Donkey F(ab')2 Anti-Goat IgG Antibody Phycoerythrin Conjugation, Donkey Fab2 Anti-Goat IgG PE Conjugated Antibody
Application:
Dot Blot, FLISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
R-PE
Concentration:
1.032 mg/ml
Extra Details:
F(ab')2 Anti-Goat IgG Phycoerythrin Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Donkey
Immunogen:
Anti-Goat IgG was produced by repeated immunization with goat IgG whole molecule in donkey.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Goat
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
Dot Blot results of Donkey F(ab')2 Anti-Goat IgG Antibody Phycoerythrin Conjugated. Dots are Goat IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: none. Secondary Antibody: Donkey F(ab')2 Anti-Goat IgG Antibody RPE at 1ug/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Rhodamine filter.
Dot Blot of F(ab')2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody. Antigen: Goat IgG. Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, or 1.23 ng as indicated. Primary antibody: N/A. Secondary antibody: F(ab')2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody at 1:1,000 for 60 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 60 min at RT.