F(Ab')2 Biotin Antibody Cy3.5 Conjugated
Product Sizes
1 mg
£663.00
700-112-098-1MG
About this Product
- SKU:
- 700-112-098
- Additional Names:
- Goat F(ab')2 Anti-Biotin CY3.5 Conjugated Antibody, Goat F(ab')2 Anti-Biotin Antibody CY3.5 Conjugation, Goat Fab2 Anti-Biotin Antibody CY3.5 Conjugation
- Application:
- Flow Cytometry, Immunofluorescence
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- Clonality:
- Polyclonal
- Conjugate:
- Cyanine 3.5
- Concentration:
- 1 mg/ml
- Extra Details:
- F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Goat
- Immunogen:
- Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
- Isotype:
- IgG
- Physical State:
- Lyophilized
- Reactivities:
- Label/Epitope-tag
- Shipping Conditions:
- Ambient
- Specificity:
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotin conjugated Goat IgG and biotin conjugated BSA. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.
- Storage Conditions:
- 2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Secondary Antibodies
- FluoroFinder:
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=700-112-098
