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Antibodies

Golden Syrian & Armenian Hamster IgG (H&L) Antibody DyLight[TM] 405 Conjugated Pre-Adsorbed

Product Sizes
100 ug
£177.00
620-146-440-100UG
About this Product
SKU:
620-146-440
Additional Names:
goat anti-Golden Syrian & Armenian Hamster IgG DyLight™405 conjugated antibody, goat anti-Hamster IgG DyLight™ 405 conjugated antibody
Application:
FLISA, Flow Cytometry, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 405
Concentration:
1 mg/ml
Extra Details:
Anti-Golden Syrian & Armenian Hamster IgG DyLight Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Goat
Immunogen:
Armenian and Golden Syrian Hamster IgG, whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Hamster
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian & Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Golden Syrian & Armenian Hamster and Golden Syrian & Armenian Hamste
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
FluoroFinder:
FYF-ITK mutant nucleofected cells display defective PLCG Gamma1 phosphorylation. (A) Thymocytes isolated from ITK-/- mice and nucleofected with cDNA constructs encoding GFP-tagged WT-, FYF-, or K390R-ITK fusion proteins, or mock-nucleofected were stimulated or not with anti-mouse CD3E Epsilon antibodies and then analyzed by flow cytometry using Alexa 647-conjugated anti-PLCG Gamma1 pY783 antibodies as described in the Materials and Methods section. Results are displayed as cell number versus fluorescence intensity. In each panel the grey dotted line histograms represent mock-nucleofected cells that were not stimulated as negative controls for setting an electronic gate (vertical line) for calculation of positive cells. Histograms of non-stimulated, nucleofected or mock-nucleofected cells were similar. The black dotted line histograms represent K390R-ITK nucleofected cells that were stimulated. The solid black line histograms represent WT-ITK nucleofected (left panel) and FYF-ITK mutant nucleofected (right panel) cells that were stimulated. The table inset lists anti-PLCG Gamma1 MFI of the displayed stimulated cell histograms. Figure 2. PMID: 23028816.