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Antibodies

Swine IgG F(ab')2 Antibody

Product Sizes
5 mg
£243.00
614-4104-5MG
About this Product
SKU:
614-4104
Additional Names:
Rabbit Anti Swine IgG F(ab')2 Antibody, Rabbit Anti-Swine IgG Fab2 Antibody, Rabbit Anti-Swine IgG Fab2 Fragment Antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
4.6 mg/ml
Extra Details:
Anti-Swine IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the Swine IgG molecule. Swine IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. F(ab')2 molecules lack the Fc portion of IgG and therefore receptors that bind Swine IgG F(c) will not bind Swine IgG F(ab')2 molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Swine IgG F(ab')2 fragment
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Porcine
Shipping Conditions:
Blue Ice
Specificity:
IgG F(ab')2|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Swine IgG, Swine IgG F(ab')2 and Swine Serum. No reaction was observed against Swine IgG F(
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
ELISA Results of Rabbit Anti-Swine IgG F(ab')2 Antibody tested against purified Swine IgG F(ab')2. Each well was coated in duplicate with 1.0 ug of Swine IgG F(ab')2 (p/n 014-0104) [Green Line] and Swine IgG (p/n 014-0102) [Blue Line]. The working dilution of Swine IgG F(ab')2 is 1:88,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Goat Anti-Rabbit IgG HRP conjugated (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).