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Antibodies

Sheep IgG (H&L) Antibody

Product Sizes
2 mg
£177.00
613-7102-2MG
About this Product
SKU:
613-7102
Additional Names:
Donkey Anti-Sheep IgG Antibody
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
2 mg/ml
Extra Details:
Sheep IgG whole molecule antibody generated in donkey detects specifically Sheep IgG whole molecule. This secondary antibody anti-Sheep is ideal for investigators who routinely perform western blot, immunoprecipitation and more generally immunoassays.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Donkey
Immunogen:
Sheep IgG whole molecule
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Sheep
Shipping Conditions:
Blue Ice
Specificity:
Anti-SHEEP IgG Antibody was prepared from monospecific antiserum generated in donkey by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.|IgG (H&L)
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
ELISA results of purified ELISA of Donkey anti-Sheep IgG Antibody tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 ug of Sheep IgG. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Gt-a-Donkey IgG HRP conjugate 1:10,000, and TMB-8000 substrate.