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Antibodies

Sheep IgG (H&L) Secondary Antibody Biotin Conjugated Pre-Adsorbed

Product Sizes
500 ug
£177.00
613-4628-0500-500UG
About this Product
SKU:
613-4628-0500
Additional Names:
Rabbit Anti-Sheep IgG biotin Conjugated Antibody, Rabbit Anti-Sheep IgG Antibody biotin Conjugation
Application:
Dot Blot, ELISA, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Biotin
Concentration:
1 mg/ml
Extra Details:
When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Sheep IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Sheep
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Pr
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Dot Bot of Rabbit Anti-Sheep IgG Biotin Conjugated Antibody Min X human serums. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Primary Antibody: Rb-anti-sheep Biotin at 1ug/mL. Secondary Antibody: Streptavidin HRP S000-03 at 1:40,000. Blocking Buffer: BlockOut MB-073 for 30 min at RT.
ELISA results of purified Rabbit anti-Sheep IgG biotin conjugated antibody Min X human serum tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 ug of sheep IgG (red line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate 1:10, 000, and TMB-1000 substrate.