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Antibodies

Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Product Sizes
1 mg
£210.00
612-6502-1MG
About this Product
SKU:
612-6502
Additional Names:
Sheep Anti-Rat IgG alkaline phosphatase Conjugated Antibody, Sheep Anti-Rat IgG Antibody alk phos Conjugation
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
AP
Concentration:
1 mg/ml
Extra Details:
Anti-Rat IgG (H&L) generated in sheep detects rat Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG is conjugated to alkaline phosphatase.
Formulation:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Host:
Sheep
Immunogen:
Rat IgG whole molecule
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Rat
Shipping Conditions:
Blue Ice
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Rat IgG and Rat Serum.
Storage Conditions:
2-8[o]C unopened. Do not freeze.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Western Blot of Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n 612-6102). Lane M: 3 ul Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n 612-6102) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody (p/n 613-703-168) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.