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Antibodies

CaM Kinase II phospho T286 Antibody

Product Sizes
100 ul
612-401-C71-100UL
About this Product
SKU:
612-401-C71
Additional Names:
Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, Camk2a protein|Camk2A/B
Application:
ELISA, Western Blot
Buffer:
0.01 M HEPES, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
CaM Kinase II antibody reacts with Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) which is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning. Anti-CaM Kinase II antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Formulation:
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Host:
Rabbit
Immunogen:
Anti-CaM Kinase II pT286 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-CaM Kinase II pT286 Antibody is directed against CaM Kinase II protein phosphorylated at T286. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulte
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit anti- CaM Kinase II Thr286 Antibody. Lane 1: rat brain lysate. Lane 2: rat brain lysate incubated in L Lambda-Ptase (1200 units for 30 min). Load: 10 ug per lane. Primary antibody: CaM Kinase II Thr286 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50kDa A Alpha- and the ~60kDa for B Beta-CaM Kinase II phosphorylated at Thr286. Other band(s): none.