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Antibodies

Rabbit IgG (H&L) Antibody DyLight[TM] 549 Conjugated Pre-Adsorbed

Product Sizes
100 ug
611-742-127-100UG
About this Product
SKU:
611-742-127
Additional Names:
Donkey Anti-Rabbit IgG Antibody DyLight 549™ Conjugated, Donkey Anti Rabbit IgG DyLight 549™ Conjugated Antibody
Application:
Detection/Quantitation, Dot Blot, FLISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
DyLight™ 549
Concentration:
1 mg/ml
Extra Details:
Anti-Rabbit IgG (H&L) DyLight 549 Antibody generated in donkey detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Donkey
Immunogen:
Rabbit IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Western Blot of Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-701-127). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), F(c) and Serum, 25 ng of IgM. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-701-127) 1:7,500 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight™ 549 conjugate. Anti-TNFa was detected using a DyLight™ 649 conjugate. The image was captured using the Typhoon™ 9410 Imaging System.
Rockland Rabbit anti-Transferrin (109-4134, lot 3033, green), Goat Anti-Alpha-1-Anti-Trypsin (100-101-147, lot 5842), and Mouse Anti-GST (200-301-200, lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween overnight at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 overnight at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127, lot 21100, shown as green), DyLight 488 Donkey anti-Mouse IgG (610-741-124, lot 21095, shown as blue), and DyLight 649 Donkey anti-Goat IgG (605-743-125, lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
Properties of DyLight™ Conjugates.