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Antibodies

Rabbit IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Product Sizes
1 mg
611-700-127-1MG
About this Product
SKU:
611-700-127
Additional Names:
Donkey anti-Rabbit IgG Antibody Rhodamine Conjugation, Donkey anti-Rabbit IgG Rhodamine Conjugated Antibody
Application:
Detection/Quantitation, FLISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
TRITC
Concentration:
1 mg/ml
Extra Details:
Anti-Rabbit IgG (H&L) Rhodamine Antibody generated in donkey detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Donkey
Immunogen:
Rabbit IgG whole molecule
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Rabbit
Shipping Conditions:
Ambient
Specificity:
IgG (H&L)|This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Secondary Antibodies
Expression of HSPB8 in MCF-7 cell lineHSPB8 mRNA and protein levels were quantified by real-time RT-PCR analysis. (C) Representative pictures of immunofluorescence staining of HSPB8 (red, anti-rabbit) and A Alpha-tubulin (green, anti-mouse) in MCF-7 cells, treated as above for 2 days. DAPI (blue) was used to stain DNA. *p<0.05 vs Control. Values represent the mean from three independent experiments. C. Control cells; E: 17B Beta-estradiol; EV: estradiol valerate; 3B Beta: 3B Beta-Adiol; Gen: genistein; Ral: raloxifen; Tam: tamoxifen. Fig 3. PMID: 28060751.
Double immunofluorescence images of glucagon-like peptide-1 receptor (GLP-1R, a, d, g) and somatostatin (SOM, b, e, h) in the pancreatic islets of chickens (a-c), northern bobwhites (d-f), and ostriches (g-i). Figures c, f, and i show merged images of a and b, d and e, and g and h, respectively. Almost every SOM-immunoreactive cell in the pancreatic islets of three avian species also demonstrated GLP-1R immunoreactivity. Bars indicate 10 um. Fig. 1. PMID: 32055175.
Immunofluorescent staining for GLP-1 (a, c) and GLP-2 (b, c') in the chicken distal ileum. a, b: Single immunofluorescent staining for GLP-1 (a) and GLP-2 (b). Both immunoreactive cells are scattered in villous epithelium and crypt of the distal ileum and show the similar localization to that indicated by double immunofluorescent staining. c, c': Double immunofluorescent staining for GLP-1 (c) and GLP-2 (c'). Most GLP-1-immunoreactive cells also show immunoreactivity for GLP-2. Bar=20 um. Fig. 1. PMID: 23759686.
Confocal microscopy imaging of particles coated by BODIPY-labeled (green fluorescence) and CD14 stained NR8383 cells (red fluorescence): (A) Cells exposed to 10 ug/cm2 DQ12 after 30 min of incubation (Left: fluorescence image; right: transmitted light image of not stained cells) (B) Unexposed cells (C) Cells exposed to 10 ug/cm2 of DQ12 after 60 min of incubation (D) Cells exposed to 10 ug/cm2 of CS after 60 min of incubation. For B, C, and D: Upper left: transmitted green fluorescence; Upper right: transmitted red fluorescence; Lower left: transmitted fused green and red fluorescence; Lower right: sham. FIG. 2. PMID: 18803060.
Distribution in the chicken distal ileum of three types of enteroendocrine cells. Enteroendocrine cell types were identified by a double immunofluorescence technique for glucagon-like peptide-1 (GLP-1) and neurotensin (NT). Arrows indicate cells showing immunoreactivity for both GLP-1 and NT (GLP-1+ /NT+ ). Arrowheads indicate cells containing either GLP-1 (GLP-1+ /NT- ) or NT (GLP-1- / NT+ ). Bars 20 um. Fig. 1. PMID: 28108848.